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Differentiation and isolation of iPSC-derived remodeling ductal plate-like cells by use of an AQP1-GFP reporter human iPSC line Authors: S Matsui, M Ochiai, K Yasuda, SI Mae, M Kotaka, T Toyoda, T Yamamoto, K Osafune Stem Cell Res, 2019;35(0):101400.

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Triple negative breast cancer (TNBC) has a poor outcome due to the lack of beneficial therapeutic targets. To clarify the molecular mechanisms involved in the carcinogenesis of TNBC and to identify target molecules for novel anticancer drugs, we analyzed the gene expression profiles of 30 TNBCs as well as 13 normal epithelial ductal cells that were purified by laser-microbeam microdissection.

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Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED 50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF 165 is approximately 1.7 x 10 3 U/μg, which is calibrated against recombinant human VEGF 165 WHO Standard (NIBSC code: 02/286).

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